Supplementary Figure 11: Transformation of Arabidopsis thaliana with the CaMV35S promoter-driven His ORFs | Nature Genetics

Supplementary Figure 11: Transformation of Arabidopsis thaliana with the CaMV35S promoter-driven His ORFs

From: Mutation of a histidine-rich calcium-binding-protein gene in wheat confers resistance to Fusarium head blight

Supplementary Figure 11

. a, Disease symptoms 4 dai of four Arabidopsis transformants observed under the fluorescent light. RE8 and RE17, plants transformed with the CaMV35S::HisR-EGFP fusion construct; R5, plant transformed with the CaMV35S::HisR construct; S11, plants transformed with the CaMV35S::HisS construct. The photos were taken by fluorescence microscope (BX53, OLYMPUS, Japan) at the 488-nm excitation wavelength. b, HisR expression in RE8, RE17 and R5. c, HisS expression in S11. d, e, Lesion areas (d) and perimeters (e) on the detached rosette leaves at 4 dai from the Arabidopsis transformants and the control Col-0. The number of inoculated leaves (n) and the P values for two-tailed Student’s t-test for comparison of the transgenic plant to Col-0 are shown. The box-and-whisker plots showed the medians (black line), upper and lower quartiles (box edges), 1.5 × interquartile range (whiskers), and outliers (dots). The number of inoculated spikes (n) and the P values for two-tailed Student’s t-test for comparison of the transgenic plants to transgene-negative siblings in each family are shown.

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