Supplementary Figure 1: Haplotype analysis using a cultivar population of 151 accessions

. Hap, haplotype; WSB, Wangshuibai; ND2419, Nanda2419; MY, Mianyang99-323; CS, Chinese Spring. The resistance levels are represented by NDS and LDR. a, Haplotypes based on gene-specific markers within the 131.6 kb (Xmag7442-Xmag9452) interval surrounding Qfhs.njau-3B and the corresponding resistance performance. 1, WSB genotype; 0, 2, 3 and 4, other non-WSB genotype. The sample size (n) is shown in column ‘No. of lines’. The P values for two-tailed Student’s t-test for comparison of Hap2 to the others are shown. b, Five haplotypes based on the 3.8-kb His-3B sequence with the main nucleotide variations shown. There are 31 and 33 SNPs and one 3-bp indel in the coding DNA sequence regions of MY and CS haplotypes compared with PH691 haplotype. c, FHB resistance of the haplotypes in b. The sample size (n) for WSB-Hap, PH691-Hap, ND2419-Hap, MY-Hap and CS-Hap is 45, 32, 16, 43 and 15, respectively. The P values for two-tailed Student’s t-test for comparison of WSB-Hap to the others are shown. In a and c, box-and-whisker plots showing the medians (black line), upper and lower quartiles (box edges), 1.5 × interquartile range (whiskers), and outliers (dots).