Supplementary Figure 2: PFT and HisR expression levels at flowering stage measured using RNA-seq data | Nature Genetics

Supplementary Figure 2: PFT and HisR expression levels at flowering stage measured using RNA-seq data

From: Mutation of a histidine-rich calcium-binding-protein gene in wheat confers resistance to Fusarium head blight

Supplementary Figure 2

. a, PFT expression in R-43 and PH691 spikes measured with FPKM. Values are represented as the means ± s.d. (n = 3 biologically independent samples). The spikelets at 0, 48 and 96 h after single-floret inoculation with F. graminearum were collected for RNA extraction. 125-bp paired reads were generated with Illumina Hiq2500. The total effective read per sample is at least 7.6 million. P values from two-tailed Student’s t-test of comparison between NIL and PH691 are shown. b, PFT expression in WSB and ND2419 spikes measured with FPKM. The spikelets at 0, 12 and 24 h after inoculation with F. graminearum were collected for RNA extraction. 150-bp paired reads were generated with an Illumina platform HiSeq X Ten System. This experiment has only one biological replicate. The total effective read per sample is at least 20 million for WSB and 22 million for ND2419. c, Expression of WSB HisR and ND2419 His-3B measured with FPKM using the same data as in b.

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