Supplementary Figure 6: Evaluation of the F1 plants derived from crosses of PH691 with three T0 plants from ND183 transformation with HisR genomic sequence
. a, Representative disease symptom 20 dai of F1 plants derived from crosses of PH691 with T0 plants #38, #40 and #45. (+) and (−), with and without transgene. b, HisR expression level relative to R-43 in the F1 transgenic plants shown in a, (n = 2 biologically independent experiments each with three technical replicates). The transgene was expressed in all the lines although the relative expression values fluctuated. c, AUDPC of the transgene-negative F1 segregants and transgenic (+) F1 segregants from the T0 plants based on NDS and LDR at 4, 8, 12, 16 and 20 dai. The number of inoculated spikes (n) and the P values for two-tailed Dunnett’s test for comparison of the transgenic (+) F1 segregants in each F1 progenies to the transgene-negative F1 segregants are shown. d, NDS and LDR at 20 dai of the transgene-negative F1 segregants and transgenic (+) F1 segregants from the T0 plants. The number of inoculated spikes (n) and the P values for two-tailed Dunnett’s test for comparison of the transgenic (+) F1 segregants in each F1 progenies to the transgene-negative F1 segregants are shown. Similar to the results from F2 analysis in Supplementary Note, NDS and LDR after 16 dai showed moderate correlation with the transgene expression level (P = 0.05). In c and d, box-and-whisker plots showed the medians (orange line), upper and lower quartiles (box edges), 1.5 × interquartile range (whiskers), and outliers (dots).
