Extended Data Fig. 7
a-d, g, Box (median and quartiles) and whisker (5-95 percentile) plots plus individual data points. a-e, In vitro differentiated primary bronchial epithelia cells (PBECs) infected with rhinovirus (RV16) or UV-irradiated control (UV-RV16) for 24 hours. a-b, Relative expression (2-ddCT) of total, long and short ACE2 in PBECs from healthy (a) (n = 13) or severe asthmatic (b) (n = 12) donors. Two-tailed Wilcoxon test UV-RV16 vs RV16. Healthy (a): total ACE2 p = 0.0010; short ACE2 p = 0.0007. Severe asthma (b) total ACE2 p = 0.0020; short ACE2 p = 0.0010. c, Ratio of short to long ACE2 expression (Δshort-long -dCT). Two-tailed Mann-Whitney test, healthy vs severe asthma UV-RV16 p = 0.0433; RV16 p = 0.0205. d, Basolateral secretion of IL29/IL28 (pg/ml) determined by ELISA from healthy (n = 14) or severe asthmatic (n = 8) donors. Unpaired two-tailed Student’s t-test, t = 2.154, df=19. Healthy vs severe asthma after RV16 infection p = 0.0433. e, Correlation of short (left) and long ACE2 (right) relative transcript expression (2-ddCT) with secreted IL29/IL28 (pg/ml) (measured by ELISA) from healthy (n = 7) or severe asthmatic (n = 5) donors Spearman’s rank correlation. f, Dot plot showing baseline expression (-dCT) of total, long and short ACE2 in bronchial epithelial brushes from healthy (n = 13) or severe asthmatic (n = 12) donors. Two-Way ANOVA, Sidak’s multiple comparisons test, F = 2.856, DF = 65.00. Total ACE2 p = 0.0013; long ACE2 p = 0.0034. g, Ratio of short to long ACE2 expression (Δshort-long -dCT) in bronchial epithelial brushes from healthy (n = 13) or severe asthmatic (n = 11) donors. Two-tailed Mann-Whitney test, healthy vs severe asthma p = 0.0474. h, Expression levels of OAS1 and ISG1 (transcripts per million (TPM) in RNAseq data) in bronchial brushings from healthy (n = 13) and severe asthmatic subjects (n = 10) Unpaired two-tailed Student’s t-test, t = 2.648, df=21, p = 0.0150. i, Correlation of short ACE2 (TPM in RNAseq data) in bronchial brushings with IFN-γ levels (pg/ml) in bronchoalveolar lavage (BAL) fluid harvested at the time of bronchoscopy. Spearman’s rank correlation r = 0.4966 p = 0.0220.
