Fig. 2: Short ACE2 is expressed in different cell types.
a, Representative agarose gel electrophoresis image of transcript-specific ACE2 RT–PCRs (n = 3) from different cell types. Size standard = NEB low-molecular-mass ladder. b, Box and whisker plots showing 5th to 95th percentiles (whiskers), median and quartiles (box) of baseline expression (−ΔCt) of total ACE2 (dark gray), long ACE2 (light gray) and short ACE2 (white) in cell lines and airway cells. Analysis was done at least in duplicate and from different passages or donors in all lines except RPE1 and HEK293 cells (analysis done in duplicate from one passage). c, Box and whisker plots showing 5th to 95th percentiles (whiskers), median and quartiles (box), plus individual data points showing relative expression (reads mapped to exon/exon boundary per million mapped reads) of short ACE2 transcript (white upward-pointing triangles) and long ACE2 transcript (gray downward-pointing triangles) in RNA-seq data from NECs at different stages of differentiation at ALI (days 1, 4, 8, 14, 28, 63 and 84; n = 3 donors for each time point) and primary ex vivo nasal brushings (n = 6 donors). d, Medians and individual data points showing baseline expression (−ΔCt) of total ACE2 transcripts, long ACE2 transcript and short ACE2 transcript in nasal (white upward-pointing triangles, n = 11 donors) and bronchial (gray downward-pointing triangles, n = 11 donors) ALI cultures from healthy donors, as determined using transcript-specific qPCR. Data were analyzed using the Mann–Whitney U-test. e, Medians and individual data points showing baseline expression (−ΔCt) of total ACE2 (dark gray upward-pointing triangles), long ACE2 (light gray circles) and short ACE2 (white downward-pointing triangles) transcript in a multiple tissue cDNA panel 1 (636742, TakaraBio), as determined using transcript-specific, probe-based ACE2 RT–qPCR. ND, not detected; n = 2 independent experiments.
