Extended Data Fig. 1 | Nature Genetics

Extended Data Fig. 1

From: A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection

Extended Data Fig. 1

a, Plots of CT value against cDNA input into RT-qPCR reactions to determine dynamic range of RT-qPCRs for 3 SyBr green assays using primers targeting total ACE2 (top left), long ACE2 (top right) and short ACE2 (bottom left) and one probe-based Taqman assay using primers targeting short ACE2. CT values below the indicated dotted line were regarded as positive results. b, Box and whisker plots showing 5-95 percentile, median and individual data points of relative expression (2-ddCT) of short ACE2 in untreated nasal ALI cultures and those infected with RV-16, UV-RV16, assayed using SyBr green qPCR assay compared to probe-based Taqman assay to show consistency of short ACE2 qPCR assays. Shapiro Wilk test of normality failed, so non-parametric Wilcoxon test used, two-tailed, comparing RV16 to UV-RV16, n = 11 different donors of nasal brushings. p = 0.001.

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