Extended Data Fig. 5
a, Coomassie stained polyacrylamide gel showing purification of short (left) and long (right) ACE2 ectodomains from an E. coli cell-free system. Tot = total lysate loading, P = purified protein. Black arrows point to purified proteins at expected sizes. b, Wide field epifluorescence and phase contrast image overlay of H441 cells transfected with exogenous long ACE2-turboGFP (left) or exogenous short ACE2-turboGFP (right). Scale bar = 50 μM. White arrows point to membrane localization of long ACE2-turboGFP.
