Extended Data Fig. 1: Extended characterization of chemically degradable MED-dTAG alleles.
From: Selective Mediator dependence of cell-type-specifying transcription
a, MED-dTAG depletion mean of two independent image quantifications of the Fig. 1b immunoblot. b, Degrader treatment selectively destabilizes the tagged Mediator subunit without affecting other complex members. c, Time-resolved immunoblot of MED10-dTAG and direct pharmacologic degradation of CDK9 (dCDK9; THAL-SNS-032) or BRD4 (dBET6). d, Pearson correlation of average 3’ mRNA-seq log2 fold changes after 6 h (n = 3 independent drug treatments). For dTAG-carrying cell lines (only gene names shown), we compare dTAG7 vs. vehicle control in the same cell line. Other conditions represent drug vs. vehicle control in wild-type cells. e, Gene ontology (GO) terms enriched among negative PC2 loadings in Fig. 1c. Enrichment was calculated using the GSEAPreranked tool75. Negative enrichment indicates a strong influence of these terms on PC2 diversity and that the underlying genes are downregulated. f, Gene set enrichment analysis of top 100 core MYC target genes from (ref. 40) among PC2 loadings. g, Time-resolved immunoblot of MED14-dTAG degradation kinetics and its influence on MYC protein levels. Unprocessed western blots shown in Source Data.
