Fig. 8: Tissue-specific genome architecture in mature brain, liver and sperm cells.
From: Three-dimensional folding dynamics of the Xenopus tropicalis genome
a, Heatmaps of chromosome 2 plotted at a 50-kb resolution for brain and liver cells. b, Hi-C matrices (oberved/expected) and Cscore for chromosome 2 at 50-kb resolution for brain and liver cells. c, Heatmaps of an example region in chromosome 2 showing the gain and loss of TAD structures in the brain, liver and sperm cells compared to s13. The single asterisk next to Liver indicates repeated Hi-C on liver cells with K562 as the spike-in control. d, Aggregated TADs of brain, liver and s13 embryo cells and normalization of aggregated TADs against s13 embryos. e, DI cluster for TADs of brain and liver cells. In brain cells, we identified 874, 3,361 and 912 TADs in clusters 1, 2 and 3, respectively. In liver cells, we identified 11, 2,469 and 5 TADs in clusters 1, 2 and 3, respectively. f, Western blot of CTCF, Rad21, SNF2H, RPB1 and histone H3 as a control in brain and liver cells. The western blot experiments in this figure were repeated at least twice with similar results. g, Heatmap of chromosome 2 plotted at a 50-kb resolution for mature sperm cells.
