Extended Data Fig. 9: Networks of KMT2A fusion target genes show divergent patterns of active and repressive chromatin marks within the same leukemia.
From: Automated CUT&Tag profiling of chromatin heterogeneity in mixed-lineage leukemia
a, Organizing genes according to the co-variance of either the imputed H3K4me3 (left) or H3K27me3 (right) scores across ML-2 cells resolves groups that vary in concert with one another from cell-to-cell but are anti-correlated with genes in the other group. Bivalent targets (Cyan) and bivalent missing targets (Magenta) are indicated. b, Same as (a) for SEM cells. c, Same as (a) for RS4;11 cells. d, Same as (a) for KOPN-8 cells. e, Same as (a) for the 10 ALL-1 cells. f, Same as (a) for the 10 AML-2 cells. g, Same as (a) for the 10 MPAL-2 cells. The ML-2, KOPN-8, 10 AML-2 and 10 MPAL-2 cells show the clearest distinction between divergent groups of oncoprotein target genes. h, Knee plot showing the distribution of the imputed HOXA9 H3K4me3 scores across all cells profiled from the 10 MPAL-1 sample. Only ~15% of cells have a log-transformed HOXA9 H3K4me3 score > 0.6. i, Same as (h) but showing imputed HOXA9 H3K27me3 scores. The majority of 10 MPAL-1 cells (~55%) have a log-transformed HOXA9 H3K27me3 score > 0.6.
