Extended Data Fig. 3: Comparison of fusion oncoprotein binding sites across all samples.
From: Automated CUT&Tag profiling of chromatin heterogeneity in mixed-lineage leukemia
a, Box plot showing the fold difference between the KMT2A-N and -C signal at wide KMT2A peaks in the control CD34 + and K562 samples is significantly less than at oncoprotein target sites in the KMT2Ar samples. Center line = median; box limits = first and third quartiles; whiskers show all data within 1.5 IQRs of the lower and upper quartiles; outliers are not shown; P values were computed using a two sample t-test (two-sided) comparing the fold difference between the KMT2A-N and -C signal on KMT2A fusion oncoprotein sites to wide KMT2A peaks in the CD34 + control; CD34 + , n = 131; K562, n = 65; ML-2, n = 144; SEM, n = 91; RS4;11, n = 92; KOPN-8, n = 192; 10 ALL-1, n = 156; 10 AML-1, n = 349; 10 AML-2, n = 423; 10 AML-3, n = 103; 10 AML-4, n = 270; 10 AML-5, n = 186; 10 MPAL-1, n = 248; 10 MPAL-2, n = 189. b, Line plot of all genes showing the number of samples in which a particular gene is called as an oncoprotein target. c, Same as (b) but only genes called as an oncoprotein target in at least one sample are included. The most frequent oncoprotein target genes are indicated.
