Extended Data Fig. 3: Cytogenetic status of seismic amplification in neuroblastoma.
From: Chromothripsis followed by circular recombination drives oncogene amplification in human cancer
(a) Confocal microscopy images of FISH analyses using distinct probes covering three hotspot regions of seismic amplification in neuroblastoma (that is, loci of MYCN, CDK4, and MDM2). Seismic amplification presented as clustered signals, indicating HSR (homogeneously staining regions) or NC (neochromosomes), or as extensively spread signals, indicating DM, both in interphase nuclei of tumor samples and in metaphase spreads of cell lines. Classification as DM, HSR or NC is indicated on respective images, if differentiation between extra- and intrachromosomal amplification was possible. Scale bars, 10 μm. CEP2 and CEP12, centromere protein of chromosome 2 and 12, respectively; XCP12, whole chromosome 12 paint. Each experiment was repeated at least two times independently. (b) Multicolor FISH (left) and corresponding G-banding (middle) of metaphase spreads of cell line NGP. White/black arrowheads indicate a HSR harboring chromosome 12 material integrated into chromosome 12; white/black asterisks indicate a HSR harboring chromosome 2 material integrated into chromosome 4. Fluorescence R-banding of metaphase spreads of cell line LS (right), corresponding to m-FISH analysis of Fig. 2f. Two neochromosomes harboring seismic amplicons are indicated by white arrowheads.
