Fig. 2: Active and repressive chromatin states in single cells from the mouse BM.
From: Single-cell sortChIC identifies hierarchical chromatin dynamics during hematopoiesis
a, UMAPs of H3K4me3 (nā=ā6,262), H3K4me1 (nā=ā6,242) and H3K27me3 (nā=ā3,452) single-cell epigenomes from whole BM (unenriched), Linā and LSK sorted populations. b, UMAPs colored by cell type. Eryths, erythroblasts; baso/eosino, basophils/eosinophils; pDCs; monocytes; HSPCs, hematopoietic stem cells and early progenitor cells. c, UMAP summary colored by sortChIC signal in a region Ā±5ākb centered at the transcription start site of Ebf1, a B-cell-specific gene. d, Same as c but for a region around S100a8, a neutrophil-specific gene. e, Heatmap of sortChIC signals for regions around cell-type-specific genes showing high levels of active marks (H3K4me1, H3K4me3) in their respective cell type, and correspondingly low levels in the repressive mark (H3K27me3). f, Example of active and repressive chromatin states near the transcription start site of a B-cell-specific TF Ebf1. H3K4me3 and H3K4me1 show large number of cuts specifically in B cells; H3K27me3 shows B-cell-specific depletion of cuts. Colored line plots (same color code as in b) represent the average sortChIC signal for cells of the same cell type. Individual cells are ordered by cell type and color-coded on the left.
