Extended Data Fig. 1: sortChIC generates high-resolution maps of histone modifications in single cells.
From: Single-cell sortChIC identifies hierarchical chromatin dynamics during hematopoiesis
(a) FACS plots for sorting individual K562 cells in G1 phase. (b) Fraction of cuts starting with TA (reflecting the preference of MNase to cut in an AT context) versus number of cuts mapped to the K562 genome. Cells below horizontal dotted lines and left of vertical lines are excluded from the analysis. (c) Distribution of fraction of cuts mapped to locations within peaks across cells. (d) Correlation between pseudobulk sortChIC and bulk ChIP signal using 50 kilobase (kb) bins for H3K4me1, H3K4me3, H3K27me3, and H3K9me3. (e) Pearson correlation between pseudobulk sortChIC and bulk ChIP signal using 50 kb bins across the four histone marks. (f) Three tracks of H3K9me3 ChIP-seq bulk data, one for H3K9me3 without normalization (H3K9me3), one for the input (Input), and one where H3K9me3 is normalized to the input (H3K9me3/input). Fourth track is H3K9me3 sortChIC pseudobulk, showing that H3K9me3 ChIP-seq requires normalizing by input to resemble sortChIC.
