Extended Data Fig. 9: BAF and HNF1A-mediated regulation of ACE2 expression and SARS-CoV-2 infection is not conserved in mice.
From: Pharmacological disruption of mSWI/SNF complex activity restricts SARS-CoV-2 infection
(a) Schematic of Comp14 SMARCA4/2 ATPase inhibitor treatment (i.p. daily at 25 mg/kg) and virus infection in C57BL/6 J mice. (b) Body weight change of mice injected with Comp14 following the protocol outlined in (a). (c, d) SARS-CoV-2 MA10 virus titers (c) and viral genome levels (d) in lung tissues at 2 dpi. (e) C57BL/6 J mice were i.p. injected with Comp12 daily at 10 mg/Kg for 5 days and Ace2 expression in different tissues was measured by RT-qPCR. (f) HNF1 motif density around the ACE2 locus in human and mouse genomes. Data in (b-d) were analyzed by one-way ANOVA with Tukey’s multiple comparison test. Shown are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, n = 7 mice, or n = 3 mice. Data in (e) were analyzed by two-tailed t-test, n = 8 mice for DMSO, n = 12 mice for Comp12.
