Fig. 3: Characterization of a wild tomato cytochrome P450 gene, Sgal12g015720.
a, A 244-bp deletion in the first exon of Sgal12g015720 in the three domesticated tomatoes. Genome coverages when mapping Illumina reads against the S. galapagense reference genome are illustrated by yellow (ten wild species) and gray (three cultivated accessions) histograms. Green lines, 5′ and 3′ UTRs; bold green lines with white arrows inside, exons; light green lines, introns; S. lycopersicum A, S. lycopersicum var. cerasiforme; S. lycopersicum B, S. lycopersicum var. lycopersicum cv. M82; S. lycopersicum C, S. lycopersicum var. lycopersicum cv. Heinz 1706. b, PCR validation of the 244-bp deletion in ten wild and three domesticated tomatoes. Three experiments were independently conducted with similar results. c, Expression levels (transcripts per million (TPM)) of Sgal12g015720 in different tissues of wild tomato S. pennellii. d, Comparison of phenotypes of the WT Micro-Tom (left panel) plant and the T2 generation of the Sgal12g015720-OE transgenic plant (right panel). Scale bar, 5 cm. e–i, Fruit number per plants (e), total fruit weight per plant (f), single fruit weight for red fruits (g), transverse diameter for red fruits (h) and longitudinal diameter for red fruits (i) in WT and T2 transgenic plants. For e and f, three independent WT and OE plants are used. In g–i, the number of fruit samples for WT is 55 and numbers of fruits for OE-1, OE-2 and OE-3 are 23, 22 and 26, respectively. Data are presented as mean ± s.d.; ***P < 0.001; **P < 0.01; *P < 0.05 in two-tailed Student’s t test.
