Fig. 1: Lr9 and Lr58 encode an unusual tandem kinase fusion protein.
From: An unusual tandem kinase fusion protein confers leaf rust resistance in wheat
a, Phenotypic effect of Lr9 and Lr58 against P. triticina. TcLr9 and Transfer represent Lr9-carrying bread wheat lines, and Thatcher is the susceptible control. TA5605 is an Lr58-carrying bread wheat backcross line in the genetic background of the susceptible parent WL711. Scale bar = 1 cm. b, Representative images showing the phenotype of susceptible TcLr9 mutants. Mutants in bold were used for MutIsoSeq. Blue and yellow boxes show magnified sections of the partially susceptible mutants M388 and M494, respectively. Scale bar = 1 cm. c, Schematic representation of MutIsoSeq. Iso-seq is used to obtain full-length transcript sequences of a WT parent. RNA sequencing reads generated from EMS mutants are mapped against the WT full-length transcripts and SNPs are called. Transcripts showing an EMS-type point mutation (red stars) in all the mutants are considered candidates (transcript 2 in this example). d, The predicted WTK6-vWA protein structure; salmon red—kinase 1, light brown—kinase 2, purple—vWA ___domain, light blue—Vwaint ___domain. Vertical bars represent 67 EMS-induced amino acid substitutions, upper bars represent surface-localized residues, lower bars represent internal residues and black stars represent premature stop codon and start codon mutations. VIGS1 and VIGS2 indicate the positions of the two VIGS constructs. e, Representative images showing the results of the VIGS experiment using barley stripe mosaic virus (BSMV). BSMV-γVIGS1 and BSMV-γVIGS2 indicate two silencing constructs targeting WTK6-vWA, BSMV-γGFP indicates control with a GFP silencing construct and BSMV-γPDS indicates silencing construct targeting the phytoene desaturase genes. Scale bar = 1 cm.
