Extended Data Fig. 6: Extended analysis of transcriptomic effects of CF-KOs.
From: In vivo screening characterizes chromatin factor functions during normal and malignant hematopoiesis
(a) Analysis of the effect of Chromatin factor disruption (CF-KOs) on lineage specific expression patterns, comprising markers and transcription factors specific for progenitor, Myeloid, Erythroid, Megakaryocytic, Basophil and B-cell lineages. The color of each dot represents the log2 fold change (compared to NTCs), the size represents the –log10 adjusted p-value, as per key to the bottom right. P-values were calculated using negative binomial mixed models from the nebula R package. All values are shown in Supplementary Table 5. (b) Gene set enrichment analysis (GSEA) of differentially expressed genes in knockouts of factors belonging to repressive complexes. The color of each dot represents normalized enrichment score, the size represents the –log10 adjusted p-value. P-values were calculated by random sampling as implemented in the fgsea R package.
