Extended Data Fig. 7: Extended analysis of effects of CF-KOs on chromatin accessibility and TF footprints.
From: In vivo screening characterizes chromatin factor functions during normal and malignant hematopoiesis
(a) MA plots demonstrating differential accessibility analysis between selected CF-KOs and Control (NTC). Up- and down-regulated genomic loci are indicated in red and blue, respectively. (lower panel) Volcano Plots showing the differentially bound TF motifs (estimated by TOBIAS) between the same CF-KOs and Control (NTC). Gained and lost footprints are indicated in red and blue, respectively, n = 2 independent experiments. (b) Time-series analysis of chromatin accessibility dynamics under ex vivo priming conditions at day 3, 5 and 7, n=2 independent experiments. (c) Effect of Kmt2d- and Wdr82-KOs on the differential accessible patterns derived from the time-series analysis. n=8436, 19015, 9383, and 9275 for all conditions and days in the mid-late, lost, late, and transient clusters, respectively (n=2). Boxplots display the median and the distribution’s 25th (minima) and 75th (maxima) percentiles. The whiskers extend up to 1.5 times the interquartile range (Q3-Q1) from the minima and maxima. (d) Time-series analysis of differentially bound TF motifs (estimated by TOBIAS) under lineage priming conditions for Kmt2d- and Wdr82-KOs.
