Extended Data Fig. 8: In vivo binding patterns of BAF and COMPASS complexes.
From: In vivo screening characterizes chromatin factor functions during normal and malignant hematopoiesis
(a) CF binding at representative loci in Myeloid (GMP) and Erythroid (MEP) progenitors. Genomic Coordinates: Cebpa chr7:35,114,878-35,131,210; Cebpe chr14:54,702,383-54,717,520; Elane chr10:79,871,207-79,893,610 ; Gfi1b chr2:28,585,038-28,624,000; Hbb chr7:103,845,151-103,886,745; Car2 chr3:14,855,264-14,912,573. (b) Heatmaps showing lineage-specific binding patterns for each CF. (c) Heatmap showing joint analysis of Smarcb1/cBAF and Brd9/ncBAF binding in Myeloid progenitors (GMPs) and in mature Myeloid cells (monocytes). This analysis shows that Smarcb1/cBAF has widespread binding at early Myeloid stages while Brd9/ncBAF exhibits more presence in mature Myeloid cells (Monocytes). Strong overlap between cBAF and ncBAF complexes seems limited to few regions. (d) Representative binding tracks of Smarcb1 and Brd9 binding in GMP and Monocytes at progenitor loci (upper panel) and mature Myeloid loci (lower panel). (e) TF motif co-occurrence in lineage specific binding patterns of Smarcb1 (cBAF), Brd9 (ncBAF), Kmt2a (MLL) and Kmt2d (MLL4). TF motifs (discovered with HOMER) are sorted by their odds ratios (y-axis) in Kmt2a- Kmt2d- Brd9- and Smarcb1- lineage specific peaks: GMP, Mye (GMP & Monocytes), MEP, Ery (MEP & Erythrocytes) and B-cells. The color scale reflects the –log10 p-adjusted values for each TF motif.
