Extended Data Fig. 10: Extended analysis of cBAF, MLL and MLL4 binding and TF-partnership in Npm1c/Flt3-ITD Leukemia.
From: In vivo screening characterizes chromatin factor functions during normal and malignant hematopoiesis
(a) Correlation (spearman) analysis of CF binding patterns in Myeloid progenitors, Mature myeloid and leukemic populations. (b-d) Enrichment analysis of Smarcb1-, Kmt2a- and Kmt2d- bound loci specific of leukemia cells. Bar graphs show enriched terms across input gene lists, sorted by p-values. Targets connected to leukemia specific peaks (nearest TSS) were run in Metascape87. Functions with particularly high relevance for Npm1c/Flt3-ITD leukemia are highlighted in bold. (e) Genome-browser snapshots showing binding of cBAF/Smarcb1, MLL/Kmt2a and MLL4/Kmt2d on leukemic specific loci. (f) MA plots showing accessibility changes after acute depletion of Smarcd2-KO (cBAF), Kmt2a-KO (MLL) and Kmt2d-KO (MLL4) with respect to Control cells harbouring NTC sgRNAs. Color coding loci overlapping with Stat5a (red), Runx1(green) and Runx2 (blue).
