Fig. 2: Relative increase in mtDNA copy number with larger cell size.
From: Single-cell mtDNA dynamics in tumors is driven by coregulation of nuclear and mitochondrial genomes
a, Distribution of single cell mtDNA copy number across all the cell lines, PDX model and tumor libraries—ordered by decreasing median mtDNA copy number. Within each library, there is a wide variation in the distribution of mtDNA copy numbers. b, Median mtDNA copy number of 184-hTERT SA039 cell line across time points p25, p30, p51 and p60 (n = 4,011 cells). Error bars indicate the s.e. of the median values. c, Same as b but for the following four different TP53−/− 184-hTERT cell lines—SA906a at time points p10, p15, p25, p30, p40, p50 and p57 (n = 4,750 cells), SA906b at time points p20, p30, p35, p40, p45, p50 and p55 (n = 6,633 cells), SA1101a at time points p10, p15, p20, p25, p30, p35, p40, p45 and p50 (n = 6,720 cells) and SA1101b at time points p15, p20, p25, p30, p35, p40, p41, p45, p50, p55 and p60 (n = 11,909 cells). d, Same as c but for the following three different TNBC PDX samples—SA1035 at time points p4, p5, p6, p7 and p8 (n = 3,243 cells), SA609 at time points p3, p6 and p10 (n = 2,401 cells) and SA501 at time points p2, p5, p6, p11 and p15 (n = 2,153 cells). e, Scatter plot showing a positive Pearson correlation (R = 0.51, P = 1.54 × 10−43) between mtDNA copy number and cell diameter for a sequencing library of a TP53−/− 184-hTERT SA906b cell line, A96155B (n = 627 cells). Contours represent a two-dimensional kernel density estimate. Gray-shaded areas represent error bands indicating the 95% confidence interval, and the red dashed line indicates the regression line. f, Same as e but for a Pearson correlation (R = 0.33, P = 1.16 × 10−19) on a sequencing library of a TNBC SA1035 PDX, A95623A (n = 704 cells). g, Dot-and-whisker plot of the Pearson correlation coefficient for every library across all the cell lines (n = 52). Consistent, positive coefficient estimation across multiple libraries of the same sample corroborates the relationship between cell size and mtDNA copy number. The dots have their colors filled in only if the library is statistically significant (false discovery rate (FDR) < 0.05). Error bars indicate 95% confidence intervals. h, Same as g but for tumors (n = 17 and 7 libraries for PDXes and tumors, respectively).
