Extended Data Fig. 1: Compartments and domains are maintained in the absence of YY1.
From: YY1-controlled regulatory connectivity and transcription are influenced by the cell cycle
a, YY1-AID cell counts for −auxin and +auxin conditions. Results are shown as mean ± s.d. (n = 3 biological replicates, two-sided independent t-test). b, Western blot for YY1 in parental cells and YY1-AID cells after an auxin time-course. c, Histogram of YY1 ChIP–seq signal at YY1 peaks for −auxin and +auxin conditions. d, ChIP–seq tracks for YY1 (Active Motif antibody), YY1 (Bethyl antibody) and H3K27ac from −auxin and +auxin conditions. e, Heatmaps of YY1 ChIP–seq signal (Bethyl antibody) and mean log2-fold change (+auxin/−auxin) centered on all YY1 peaks (n = 2 biological replicates). f, Micro-C contact versus distance curves for each biological replicate. g, Heatmap of Pearson correlations of compartment eigenvector 1 values (EV1) between biological replicates. h, Micro-C contact maps from −auxin (top) and +auxin (bottom) conditions along with tracks of compartment EV1, with positive values corresponding to A compartment and negative values corresponding to B compartment. i, Histogram of EV1 values from −auxin and +auxin contact maps (n = 25,257 bins). j, Aggregate ___domain plot for all domains called in the −auxin contact map, centered on upstream and downstream boundaries. k, Histogram of log2 insulation score values from −auxin and +auxin contact maps for all boundaries called across both conditions. l, Histogram of log2 insulation score values from −auxin and +auxin contact maps for the subset of boundaries that have YY1 binding within ±50 kb. m, Micro-C contact maps from −auxin (top) and +auxin (bottom) conditions, annotated with example TAD calls. Corresponding tracks show log2 insulation scores (IS).
