Extended Data Fig. 9: scRNA-seq and CyTOF analysis reveal increased immune cell infiltration in Men1 knockout CT26 tumors, related to Fig. 6.
(a) Schematic view of CT26 tumor scRNA-seq and CyTOF experiments. (b) Inferred copy number (CNA) for all the cells. Red indicates copy number gain and blue indicates copy number loss. (c) UMAP view of single cells from scRNA-seq profiling, color coded by tumor samples with (red) or without (blue) deletion of Men1. (d) Bar plot showing the differences of the number of cells in each sub-clusters in Men1-knockout versus control tumor samples from scRNA-seq profiling. (e) Heatmap showing the marker gene expression in each sub-clusters. (f) UMAP view of single cells from CyTOF profiling, color coded by tumor samples with (red) or without (blue) deletion of Men1. (g) Bar plot showing the differences of the number of cells in each sub-clusters in Men1-knockout versus control tumor samples from CyTOF profiling. (h) Tumor growth rate of A549 xenograft in humanized mice with and without knockout of MEN1. Each data point represents mean ± s.e.m. tumor volumes (n = 5 in sgCtrl and sgMEN1 group). Two-way ANOVA test was used for the statistical test of the growth curves. ****: p value <0.0001. (i) Bar plot showing the percentage of infiltrated CD8+ T cells in tumors with and without knockout of MEN1. Mean ± s.d. of quantifications from 6 and 8 IHC sections for sgCtrl and sgMEN1biological replicates were shown for each measurement (unpaired two-tailed Student's t-test). *: p value <0.05. (j) Representative images of CD8+ T cell staining in tumors with and without knockout of MEN1.
