Extended Data Fig. 4: Depot-specific correlations between estimated percentages of classical or non-classical adipocytes and clinical parameters.
Percentages of classical and non-classical adipocytes in each depot separately were estimated in an independent set of samples analyzed by bulk RNA-seq (n = 73), using sNucConv deconvolution algorithm (a validated deconvolution tool for human adipose tissue based on paired samples sequenced by both bulk and snRNA-seq – see Methods for more detail). The percentage of classical adipocytes in hSAT was negatively correlated with waist-to-hip ratio (WHR) and HOMA-IR (Spearman r = -0.4 and -0.55, adjusted p = 0.17 and 0.06, respectively). The percentage of non-classical adipocytes in hSAT was positively correlated with WHR and HOMA-IR (Spearman r = 0.34 and 0.6, adjusted p = 0.17 and 0.05, respectively). The percentage of classical adipocytes in hVAT was negatively correlated with decease in triglycerides (TG) post bariatric surgery (BS) (Spearman r = 0.49, adjusted p = 0.1). Shaded grey areas mark a confidence interval of 0.95. P-values were adjusted using Benjamini-Hochberg procedure.
