Extended Data Fig. 2: Ensuring specificity of fluorescent signal in immunofluorescence analyses of non-classical adipocytes. | Nature Genetics

Extended Data Fig. 2: Ensuring specificity of fluorescent signal in immunofluorescence analyses of non-classical adipocytes.

From: Human subcutaneous and visceral adipocyte atlases uncover classical and nonclassical adipocytes and depot-specific patterns

Extended Data Fig. 2

A-E. Representative images showing tissues expressing the specific marker used as a positive control (PTPRB – stomach (A); PDE4D – lymph node (B); SKAP1 – lymph node (C); ANK2 – kidney (D); MT-CO2 - colon (C)), no-primary antibody as a negative control, and the positive signal from within stained adipose tissue. The fluorescence emission spectra obtained from these slides are displayed in the graphs. White squares indicate the areas where spectrum measurements were taken. Shown are results of a single experiment per antibody. F. Images highlighting the presence of crown-like structures (CLS) within adipose tissue, visualized using immunofluorescence staining for PLIN1 (green), PDE4D (red) and DAPI (blue). CLS in adipose tissue act as an intrinsic positive control for PDE4D staining, displaying weak signal for PLIN1, accompanied by robust DAPI staining.

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