Extended Data Fig. 2: Spatial localization of dLum cell subsets.

a, Multicolor IF staining of a slice surrounding the urethra, related to Fig. 2d. Experiment has been repeated three times. b, IHC staining of selected markers on glandular adjacent to the urethra (top) and in the distal acini (bottom). Experiment has been repeated three times. c, Overview of the sampling strategy of the prostate of patient 2. d, H&E staining (left) and spatial transcriptomic slice, V1_5 of patient 2, colored by inferred cell proportions (right). e, IHC staining of LTF on a coronal plane of human prostate. Experiment has been repeated three times. f, UMAP of epithelial cells in scRNA-seq data colored by gene expression levels. g, Heatmap showing cell fractions of each subset of epithelial cells in scRNA-seq data (left). Cell fractions were transformed into row z score. Bar plot of cell fraction in each sample was shown on the right. h, Spatial transcriptomic slice, V1_5 of patient 2, colored by expression level of CNMD (left) and KLK5 (right), respectively. i, Pie plot showing the proportion of spots detected d6_dLum-CNMD signature in glandular ducts and other epithelial spots, respectively. P value was calculated by two-side Fisher’s exact test. j, Bar plot showing enriched pathway in d7_dLum-SPIB. T values were calculated by comparing GSVA scores per cell in d7_dLum-SPIB to all other dLum cells. k, Violin plot of expression of MHC molecules in dLum cell subsets.