Extended Data Fig. 7: Stereochemistry of AHOAP.
From: Cryptic phosphorylation in nucleoside natural product biosynthesis
Coupling constants for possible stereochemistry of C4′, C5′, C6′ and C7′. Experimental evidence of JH3′-H4′ = 10.7 Hz, JH4′-H5′ = 3.5 Hz, JH5′-H6′ = 3.5 Hz and JH6′-H7′ = 2.1 Hz for AHOA is most consistent with the stereochemistry of 4′R, 5′R, 6′R, 7′S, indicating that AHOA and AHOAP have 4′R, 5′R, 6′R, 7′S stereochemistry. Dihedral angles were calculated with ChemDraw Professional v19.0 and ChemDraw 3D v19.0 (PerkinElmer Informatics).
