Extended Data Fig. 1: Activity of PolD and PolK with 5′-OAP.
From: Cryptic phosphorylation in nucleoside natural product biosynthesis
Shown are HPLC chromatograms at 260 nm for a full reaction with PolK, a full reaction with PolD, a control without enzyme, and a control without α-KG. No consumption of 5′-OAP was detected. The results were reproducible in at least two independent assays.
