Extended Data Fig. 2: Thanatin binding efficiency and effect on Re-LPS binding.
From: Dynamics of an LPS translocon induced by substrate and an antimicrobial peptide
a, Mass spectra recorded for solutions of LptDE (5 μM) with increasing concentrations of thanatin (green adducts). b, Mass spectra of Re-LPS-bound LptDE supplemented with 1 μM thanatin (as described in Fig. 1d) with a focus on the 16+ charge state showing the presence of the 2:1 Re-LPS:thanatin complex, particularly at higher Re-LPS concentration. c, Quantification of the total amount of Re-LPS bound to LptDE in the absence or in the presence of thanatin (related to Fig. 1d). Error bars represent s.d. (n = 3). d, Thanatin-first nMS analysis: LptDE (5 μM) was initially mixed with thanatin and then supplemented with Re-LPS (10 μM).
