Extended Data Fig. 1: Sequence alignment of FEM1 proteins and C-degrons, respectively.
From: Molecular basis for arginine C-terminal degron recognition by Cul2FEM1 E3 ligase
a, Sequence alignment of human FEM1C (NP_064562.1), FEM1A (NP_061178.1), and FEM1B (NP_056137.1). The secondary structures of FEM1C and FEM1B are labelled at the top and bottom of the sequences, respectively. In the structure of SIL1-bound FEM1C, R-1 and K-4 binding residues of FEM1C, as well as their counterparts in FEM1B, are labelled in blue and orange, respectively. Their counterparts in FEM1B are also labeled accordingly. b, Sequence alignment of Arg/C-degrons, including those of SIL1, NS11, OR51B2, Clone13, and CDK5R1. The residues at -1 and -4 positions of SIL1 and their counterparts are marked.
