Extended Data Fig. 9: Changes in chromatin accessibility induced in keratinocytes following low-dose exposure to BETi are reversible upon compound washout.
From: BET bromodomain inhibitors regulate keratinocyte plasticity
Genomic loci found differentially accessible by ATAC-seq in keratinocytes upon 6 h treatment with NVS-BET-1 (125 nM) compared to DMSO. Differential peaks were calculated with DiffBind using abs(log2FC) > 1 & p-value < 0.00001. Each row represents scaled and centered accessibility values across the different replicates shown in columns for DMSO and NVS-BET-1 at 6 h. Each row represents the normalized ATAC-seq signal across three different replicates per condition shown in columns. Differences between NVS-BET-1 and DMSO at 6 h are contrasted with accessibility values for NVS-BET-2 at 6 h and with accessibility values for all conditions after 42 h of compound washout (6m48h). First side heat map on right depicts differential signal (log2 fold change) per locus for 6 h NVS-BET-1 (125 nM) versus DMSO treatment. Second side heat map on far right provides ChromHMM annotation per genomic locus (row) according to ENCODE NHEK.
