Extended Data Fig. 10: Acute skin wound healing after BETi treatment.
From: BET bromodomain inhibitors regulate keratinocyte plasticity
(a) Histological sections of mouse wounds 5 days after wounding. Samples were stained with H&E: Initial wound edge shown by a black triangle versus new wound edge indicated by a gray triangle were assigned to measure wound closure. All panels show re-epithelialization at Day 5. Top panels show 2 wounds after vehicle treatment on Day 1 and Day 3, middle panels show two wounds after two treatments with NVS-BET-1 at 3 mg/kg on Day 1 and Day 3 and the bottom panels show 2 wounds after two treatments with NVS-BET-2 at 1 mg/kg on Day 1 and Day 3. (b) Middle panel. Histological section of a mouse wound 5 days after wounding defining the areas termed epithelial tongue and wound edge. Sections from n = 5 wounds/group were stained by immunocytochemistry for Ki67, scanned and then analyzed by ImageJ 14.8 V with Immunoratio plugin. 2 images/wound were analyzed for the wound edge and up to 3 images/wound analyzed for epithelial tongue. Data shown represents image analysis for epithelial tongue (left) 0 mg/kg (vehicle) n = 10, NVS-BET-1 3 mg/kg n = 13 and NVS-BET-1 10 mg/kg (n = 10) and wound edge (right), n = 10 for all groups. The box extends from the 25th to 75th percentiles, center line shows median, and whiskers representing minimal and maximal value.
