Extended Data Fig. 7: Structural and biochemical analysis of the Cas12g-sgRNA-target RNA complex.
From: Cryo-EM structure of the RNA-guided ribonuclease Cas12g
a-b, Structural elements involved in crRNA-target RNA duplex recognition. Three such structural elements are indicated by red lines, including 1) an N-terminal extension enriched in arginines within the WED ___domain, 2) a loop within the REC2 ___domain (608-AKKATLQP-615), and 3) REC1220–354. c, Substrate RNA cleavage assay using wild-type Cas12g and Cas12g with mutations in two loops involved in the recognition of the crRNA-target RNA duplex. The results shown are representative of three experiments. d-e, Comparison of the REC1 and REC2 domains for substrate recognition between Cas12g and Cas12b.
