Extended Data Fig. 8: P4B induced ternary complexes in cells.
From: Functional characterization of a PROTAC directed against BRAF mutant V600E
a, A375 cells ectopically expressing flag-tagged CRBN/DDB1 were treated with P4B or BI 882370* at the indicated experimental conditions. Flag-tagged CRBN was immunoprecipitated using anti-Flag magnetic beads. Lysates and immunoprecipitates were subjected to immunoblot analysis with the indicated antibodies. * indicates nonspecific signal. Data are representative of two independent experiments. b. GFP-fluorescence images of HEK293T cells expressing CRBN-EGFP-HO-Tag6 and BRAF-EGFP-HO-Tag3 (WT or V600E) at the indicated time point after treatment with 0.5 µM of P4B, BI 882370* and P4BME or equivalent volume of DMSO. Data are representative of two independent experiments. Scale bars represent 10 µm.
