Extended Data Fig. 1: Structure of the BRAF kinase ___domain bound to P4B.
From: Functional characterization of a PROTAC directed against BRAF mutant V600E
a, Stereo-view of an unbiased |Fo-Fc| electron density map contoured at 2.5σ colored green with P4B and the BRAF kinase ___domain colored orange and blue, respectively. b, Stereo-view of the superimposition of P4B (orange) bound to BRAF (dark blue) and BI 882370 (light green) bound to BRAF (light blue) (PDB of BRAF:BI 882370 structure is 5CSX). c, d, Comparison of the binding mode of P4B (c) and BI 882370 (d) to the BRAF kinase ___domain. P4B, BI 882370, glucose and the BRAF contacting residues are shown as sticks. e, f, Flat schematic representation of the binding mode of P4B (e) and BI 882370 (f) to the BRAF kinase ___domain. Blue eyelashes represent hydrophobic interactions and yellow dashed lines represent hydrogen bonds.
