Extended Data Fig. 6: Characterization of mouse iPSC lines that were generated with Oct6, Oct7, Oct8, Oct9 and Oct11 under S/CI/RN treatment.
From: Permissive epigenomes endow reprogramming competence to transcriptional regulators
a, All iPSC lines were stained positive for Oct4-GFP, NANOG and SSEA1. The mO6-1 and mO6-2 lines were generated with O6SKM; the mO7-1 and mO7-2 lines were generated with O7SKM; the mO8-1 and mO8-2 lines were generated with O8SKM; the mO9-1 and mO9-2 lines were generated with O9SKM; and the mO4 line was generated with O4SKM. b, iPSC lines produced chimeras as determined by PCR. C, corresponding iPSC lines were used as positive controls. H2O was used as a negative control. L, DNA ladder. c, Karyotype analysis demonstrated correct chromosome content and no obvious deletions or duplications in the iPSC lines. d, iPSC lines formed teratomas consisting of all three embryonic germ layers, providing evidence for in vivo differentiation potential. e, mO11-1 and mO11-2 lines, which were generated with O11SKM, were stained positive for Oct4-GFP, NANOG and SSEA1. f, mO11-1 and mO11-2 lines formed teratomas. g, All iPSC lines displayed gene expression profiles similar to mESCs but distinct from MEFs. h, No Oct4 transgene was detected in mO11-1 and mO11-2 lines. Instead, Oct11 transgene was integrated in these iPSC lines. i, mO11-1 and mO11-2 lines produced chimeras as determined by PCR. C, the mO11-1 iPSC line was used as a positive control. H2O was used as a negative control. L, DNA ladder. Scale bar, 250 μm (a,e), 100 μm (d,f). The experiments were repeated independently three times with similar results and representative images are shown (a,b,d,e,f,h,i). See Source Data for uncropped blot images.
