Extended Data Fig. 2: shRNA-mediated DOT1L inhibition enables reprogramming with OCT7, OCT8 and OCT9.
From: Permissive epigenomes endow reprogramming competence to transcriptional regulators
a, Knockdown efficiency of two shRNA targeting DOT1L mRNA was determined by qPCR. The shLuc construct was used as a control. P values were 0.0006, 0.0024 and 0.0009 from left to right. b, shRNA-mediated DOT1L inhibition resulted in a dramatic reduction of H3K79me1/2 levels. c, shRNA-mediated DOT1L inhibition not only dramatically enhanced OCT4- and OCT6-based reprogramming but also enabled iPSC generation in O7SKM-, O8SKM- and O9SKM-transduced cells. P values were 0.0005, 0.007, 0.0046, 0.0011, 0.0037, 0.0038, 0.0267, 0.004, 0.0037, 0.0081, 0.0004, 0.0023, 0.0026, 0.0003, 0.0011 from left to right. Data are presented as mean values ± s.d. (a,c, n = 3 biologically independent samples). Differences between samples were compared using a two-tailed Student’s t-test (***P < 0.001, **P < 0.01, *P < 0.05). The experiments were repeated independently three times with similar results and representative images are shown (b). See Source Data for uncropped blot images.
