Extended Data Fig. 1: Cryo-EM analysis of rRyR1 in complex with Chlorantraniliprole.

Cryo-EM analysis of rRyR1 in complex with Chlorantraniliprole. a, Elution profile of rRyR1 by gel-filtration chromatography. The left inset shows the plotted standard curve for this column. The molecular weight (MW) estimated from its elution volume is ~ 2540 kDa, suggesting tetrameric form in solution (predicted MW of one subunit is 576.8 kDa). b, A 4–15% gradient SDS-PAGE showing protein marker (PM) in the left lane and purified rRyR1 and FKBP12.6 in the right lane. c, Global reconstruction of the Ca²⁺/Caf/ATP/CaM1234/CHL dataset and focus refinement mask colored by local resolution from highest resolution (3.2 Å, blue) to lowest resolution (7.2 Å, red). Insets highlight the CHL binding site and local resolution. d, Masked and corrected Fourier shell correlation (FSC) curves for Ca²⁺/Caf/ATP/CaM1234/CHL dataset calculated from half maps obtained from refinement in RELION (blue), PHENIX map density modified (DM) with Resolve (red), and FSC curved obtained from focused refinement using Mask I (transmembrane mask) as a reference with symmetry expanded particles (gold). e, Similar masked and corrected FSC curves for Ca²⁺/CHL dataset (green), PHENIX, Resolve density modified and sharpened map (purple), and for the Ca²⁺/DMSO control dataset (orange).