Extended Data Fig. 7: Relative GFP fluorescence comparison and in vitro dtRNA-regulated aptamer assay.
From: Predictable control of RNA lifetime using engineered degradation-tuning RNAs
a, Relative GFP fluorescence comparison among circuits regulated by the same dtRNAs in vitro and in vivo. Data represent the mean ± s.d. of at least three biological replicates. b, Aptamer fluorescence measurement assay. c, Comparison between in vivo relative GFP fluorescence and relative aptamer fluorescence in cell-free expression system. The result shows little correlation between relative GFP and aptamer fluorescence. Interestingly, dtRNAs with short stem-loop hairpins tend to exert stronger positive effect on aptamer fluorescence (green dots). d, Aptamer fluorescence measurements with varying 5’ single-stranded length.
