Extended Data Fig. 2: Fluorescence measurements on synthetic dtRNAs with inserted RNase E cleavage sites.
From: Predictable control of RNA lifetime using engineered degradation-tuning RNAs
a, Fifteen synthetic dtRNAs are designed without (-) or with single/multiple RNase E cleavage sites (UCUUCC) engineered into different structural regions of the stable dtRNA. The inserted regions are marked yellow (right). Fluorescence measurement result shows that insertion of cleavage sites have minor effects on RNA stability. b, Fluorescence measurement for dtRNAs with multiple RNase E cleavage sites inserted into 18-nt loop region. c, Characterize the effect of dtRNA 5’ spacing length on GFP expression. Seven dtRNAs with 5’ spacing lengths from 1-nt to 18-nt are designed to measurement their effect on GFP expression. d, Fluorescence measurement of dtRNAs with RNase E cleavage sites engineered into 12-nt 5’ spacing region. All data represent the mean ± s.d. of six biological replicates.
