Extended Data Fig. 4: qPCR measurement and dtRNAs function prediction.
From: Predictable control of RNA lifetime using engineered degradation-tuning RNAs
a, RT–qPCR measurement of relative RNA levels for dtRNAs with diverse stabilizing efficiency. The result displays a strong correlation between relative RNA levels and relative GFP fluorescence (R2 = 0.9406). Data represent the mean ± s.d. of at least three biological replicates. b, Relative fluorescence comparison between predicted relative GFP and observed relative GFP of circuits constructed followed by combined design rules (Supplementary Table 3). N is the total number for 54 single measurement regulated by additional designed dtRNAs (R2 = 0.5005). c, Fluorescence measurement of dtRNA design f (Supplementary Table 3) without (left) or with (right) 18 nt 5’ spacing. Data represent the mean ± s.d. of six biological replicates. d, Scatter plot reveals that structure MFE is not significantly correlated with GFP fluorescence enhancement regulated by synthetic dtRNA library (R2 = 0.000068). Data represent the mean ± s.d. of six biological replicates.
