Extended Data Fig. 7: PAIR1 and KIRA9 equipotently block ER stress-induced IRE1α autophosphorylation.
From: ATP-competitive partial antagonists of the IRE1α RNase segregate outputs of the UPR
Immunoblots for total IRE1α (bottom) from parent INS-1 cells treated with various concentrations of PAIR1 or KIRA9 followed by the addition of 200 ng/μL Brefeldin-A (BFA) for 2 hours. Samples were subjected to phostag SDS-PAGE and % non-phosphorylated IRE1α (top) was determined from the ratio of dephosphorylated IRE1α relative to total IRE1α. Values shown are the mean of n = 3 biologically independent samples ± SEM.
