Extended Data Fig. 2: KIRA8 completely suppresses XBP1 splicing in ER-stressed INS-1 cells.
From: ATP-competitive partial antagonists of the IRE1α RNase segregate outputs of the UPR
(a) Quantification of spliced XBP1 in untreated parent INS-1 cells and parent INS-1 cells treated with brefeldin A (BFA) or BFA and KIRA8. Values shown are the mean of n = 3 biologically independent samples ± SEM. (b) Representative example of an EtBr-stained agarose gel of XBP1 cDNA amplicons from INS-1 cells subjected to the conditions described in (a). (c) Quantification of spliced XBP1 in untreated parent INS-1 cells and parent INS-1 cells treated with thapsigargin (Tg) or Tg and KIRA8. Values shown are the mean of n = 3 biologically independent samples ± SEM. (d) Representative example of an EtBr-stained agarose gel of XBP1 cDNA amplicons from INS-1 cells subjected to the conditions described in (c).
