Extended Data Fig. 2: Sequence and structural analysis of AsCas12f1 and its mutations in the RuvC active site. | Nature Chemical Biology

Extended Data Fig. 2: Sequence and structural analysis of AsCas12f1 and its mutations in the RuvC active site.

From: Programmed genome editing by a miniature CRISPR-Cas12f nuclease

Extended Data Fig. 2

a, Multiple sequence alignment of Cas12 nucleases. b, Structure model of AsCas12f1 was built using the cryo-EM structure of Cas14a1/sgRNA/dsDNA (PDB ID: 7C7L) as a template using SWISS-MODEL web server with its default parameters. c, D225A and E324A completely abolished the nuclease activity of AsCas12f1, whereas R383A and D401A retained minor NTS nickase activity at 18-nt downstream the PAM.

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