Extended Data Fig. 4: Flow cytometry gating strategies. | Nature Chemical Biology

Extended Data Fig. 4: Flow cytometry gating strategies.

From: Programmed genome editing by a miniature CRISPR-Cas12f nuclease

Extended Data Fig. 4

a, b, Gates used for the analysis of flow cytometric data shown in Fig. 3c. Gate 1 is used to remove the debris from the cell population, gate 2 specifies single cells from cell aggregates, and gate 3 identifies the cells with GFP fluorescence. Gates were set on the control sample (a) and was then kept constant for the experimental group (b).

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