Extended Data Fig. 6: EB1 Lys66 crotonylation regulates microtubule stability.
From: Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis
(a) Multi-alignment of EB1 from different species, and the conserved K66 site of EB1 was labeled by red triangle. (b) Overall structure of Fig. 3a. (c) Cartoon drawing of in vitro microtubule plus-end-tracking assay for EB1 using TIRFM. (d) Quantification of contact number of β-tubulin and EB1 wild type and mutants in 0.1 ns. For each group, n = 1,000. Data represent mean ± s.e.m. from three independent experiments. Ordinary one-way ANOVA followed by Tukey’s post hoc test was used to determine statistical significance (***p < 0.0001). (e) The contacts number revolution in All-atom molecular dynamics simulations of EB1 wild type and mutants for 100 ns. (f) The last conformation of All-atom molecular dynamics simulation in EB1 wild type and mutants. The conformation is colored by light blue (α-tubulin), pale green (β-tubulin) and orange (EB1). Lys66 (light pink) and Glu386 (green) are showed by CPK model. Related to Fig. 3 & Supplementary table 1.
