Extended Data Fig. 8: KO cells expressing WT legumain or legumainC219S exhibit similar NF-κB activity and IL-8 expression during H. pylori infection.
From: An infection-induced oxidation site regulates legumain processing and tumor growth
(a) Luminescence of H. pylori-infected (H. pylori G27MA, MOI 25, 18 h) and uninfected KO cells transduced with WT LGMN or LGMNC219S and transiently transfected with the NF-κB-inducible luciferase reporter pNiFty2-Luc. (b) RT-qPCR analysis of IL-8 expression normalized to GAPDH expression in H. pylori-infected (H. pylori G27MA, MOI 25, 18 h) and uninfected KO cells transduced with WT LGMN or LGMNC219S. Data represent three independent experiments. Each circle represents an independent experiment. Bars represent means ± SD. n.s., not significant. Two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test was used in (a); two-tailed unpaired t-test was used in (b).
