Extended Data Fig. 10: Supplementary data for Fig. 4.
From: An infection-induced oxidation site regulates legumain processing and tumor growth
(a) Western blot analysis of legumain in two independently derived clonal lines of KO cells transduced with WT LGMN (WT#1 and WT#2) or LGMNC219S (C219S#1 and C219S#2). Western blot analysis was performed three times with consistent results. (b) Cell viability of WT AGS cells and WT#1, WT#2, C219S#1, and C219S#2 cells quantified by alamarBlue fluorescence. Data represent three independent experiments. Error bars represent means ± SD. (c) Average tumor volume of Rag2−/− IL2RG−/− mice subcutaneously implanted with KO cells transduced with WT LGMN or LGMNC219S. These data represent a second independent experiment performed using n = 12 mice and a single clonal cell line per condition (WT#1 and C219S#1). Error bars represent means ± SEM. ****P < 0.0001 by two-tailed unpaired t-test. (d) Survival curve of mice in (c). Tick marks represent censored events. ****P < 0.0001 by Mantel-Cox test.
